BMRB: 10266

Solution structures of the fn3 domain of human receptor-type tyrosine-protein phosphatase F

Authors

Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Receptor-type tyrosine-protein phosphatase F

Entity

1. Receptor-type tyrosine-protein phosphatase F (polymer, Thiol state: not present), 103 monomers, 11139.28 Da Detail

GSSGSSGPSG FPQNLHVTGL TTSTTELAWD PPVLAERNGR IISYTVVFRD INSQQELQNI TTDTRFTLTG LKPDTTYDIK VRAWTSKGSG PLSPSIQSRT MPV

Formula weight

11139.28 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 96.4 %, Completeness (bb): 94.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (1109 of 1151)	97.0 % (576 of 594)	96.0 % (433 of 451)	94.3 % (100 of 106)
Backbone	94.4 % (568 of 602)	94.7 % (196 of 207)	94.3 % (283 of 300)	93.7 % (89 of 95)
Sidechain	98.6 % (634 of 643)	98.2 % (380 of 387)	99.2 % (243 of 245)	100.0 % (11 of 11)
Aromatic	97.3 % (72 of 74)	97.3 % (36 of 37)	97.1 % (34 of 35)	100.0 % (2 of 2)
Methyl	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (58 of 58)

1. Fibronectin type III domain

GSSGSSGPSG FPQNLHVTGL TTSTTELAWD PPVLAERNGR IISYTVVFRD INSQQELQNI TTDTRFTLTG LKPDTTYDIK VRAWTSKGSG PLSPSIQSRT MPV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.95 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EDY, Strand ID: A Detail

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Release date

2009-12-13

Citation

Solution structures of the fn3 domain of human receptor-type tyrosine-protein phosphatase F
Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Receptor-type tyrosine-protein phosphatase F, : 1 : 2 : 741 entities Detail

Interaction partners 1. Receptor-type tyrosine-protein phosphatase F, : 10 interactors Detail

Experiments performed 2 experiments Detail