BMRB: 10267

Solution structures of the PDZ domain of mus musculus PDZ domain-containing protein 1

Authors

Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

PDZ domain-containing protein 1

Entity

1. PDZ domain-containing protein 1 (polymer, Thiol state: all free), 114 monomers, 12508.82 Da Detail

GSSGSSGMAS TFNPRECKLS KQEGQNYGFF LRIEKDTDGH LIRVIEEGSP AEKAGLLDGD RVLRINGVFV DKEEHAQVVE LVRKSGNSVT LLVLDGDSYE KAVKNQVDLK ELDQ

Formula weight

12508.82 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 96.4 %, Completeness (bb): 95.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (1247 of 1293)	96.0 % (648 of 675)	97.2 % (482 of 496)	95.9 % (117 of 122)
Backbone	95.1 % (647 of 680)	94.1 % (224 of 238)	95.8 % (316 of 330)	95.5 % (107 of 112)
Sidechain	97.8 % (699 of 715)	97.0 % (424 of 437)	98.9 % (265 of 268)	100.0 % (10 of 10)
Aromatic	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. PDZ domain

GSSGSSGMAS TFNPRECKLS KQEGQNYGFF LRIEKDTDGH LIRVIEEGSP AEKAGLLDGD RVLRINGVFV DKEEHAQVVE LVRKSGNSVT LLVLDGDSYE KAVKNQVDLK ELDQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PDZ domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EDZ, Strand ID: A Detail

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Release date

2009-12-13

Citation

Solution structures of the PDZ domain of mus musculus PDZ domain-containing protein 1
Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. PDZ domain-containing protein 1, : 1 : 3 : 99 entities Detail

Interaction partners 1. PDZ domain-containing protein 1, : 11 interactors Detail

Experiments performed 2 experiments Detail