BMRB: 10268

Solution structures of the CA domain of human protocadherin 9

Authors

Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Protocadherin-9

Entity

1. Protocadherin-9 (polymer, Thiol state: not present), 114 monomers, 11956.07 Da Detail

GSSGSSGNDN RPVFKEGQVE VHIPENAPVG TSVIQLHATD ADIGSNAEIR YIFGAQVAPA TKRLFALNNT TGLITVQRSL DREETAIHKV TVLASDGSST PARATVTINV TDVN

Formula weight

11956.07 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.9 %, Completeness (bb): 98.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.9 % (1193 of 1219)	96.6 % (600 of 621)	99.4 % (474 of 477)	98.3 % (119 of 121)
Backbone	98.4 % (663 of 674)	96.6 % (224 of 232)	99.7 % (332 of 333)	98.2 % (107 of 109)
Sidechain	97.7 % (635 of 650)	96.7 % (376 of 389)	99.2 % (247 of 249)	100.0 % (12 of 12)
Aromatic	92.0 % (46 of 50)	92.0 % (23 of 25)	92.0 % (23 of 25)
Methyl	100.0 % (152 of 152)	100.0 % (76 of 76)	100.0 % (76 of 76)

1. CA domain

GSSGSSGNDN RPVFKEGQVE VHIPENAPVG TSVIQLHATD ADIGSNAEIR YIFGAQVAPA TKRLFALNNT TGLITVQRSL DREETAIHKV TVLASDGSST PARATVTINV TDVN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CA domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.49 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EE0, Strand ID: A Detail

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Release date

2009-12-13

Citation

Solution structures of the CA domain of human protocadherin 9
Sato, M., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Protocadherin-9, : 1 : 4 : 1327 entities Detail

Interaction partners 1. Protocadherin-9, : 1 interactors Detail

Experiments performed 2 experiments Detail