BMRB: 10269

Solution structures of the Chromo domain of human chromodomain helicase-DNA-binding protein 4

Authors

Sato, M., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Chromodomain helicase-DNA-binding protein 4

Entity

1. Chromodomain helicase-DNA-binding protein 4 (polymer, Thiol state: not present), 64 monomers, 7676.401 Da Detail

GSSGSSGKPE WMMIHRILNH SVDKKGHVHY LIKWRDLPYD QASWESEDVE IQDYDLFKQS YWNH

Formula weight

7676.401 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.2 %, Completeness: 93.5 %, Completeness (bb): 91.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.5 % (732 of 783)	93.2 % (381 of 409)	94.4 % (286 of 303)	91.5 % (65 of 71)
Backbone	91.6 % (348 of 380)	90.0 % (117 of 130)	93.1 % (175 of 188)	90.3 % (56 of 62)
Sidechain	95.2 % (441 of 463)	94.6 % (264 of 279)	96.0 % (168 of 175)	100.0 % (9 of 9)
Aromatic	90.9 % (100 of 110)	89.1 % (49 of 55)	92.2 % (47 of 51)	100.0 % (4 of 4)
Methyl	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)

1. Chromo domain

GSSGSSGKPE WMMIHRILNH SVDKKGHVHY LIKWRDLPYD QASWESEDVE IQDYDLFKQS YWNH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Chromo domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2EE1, Strand ID: A Detail

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Release date

2009-12-13

Citation

Solution structures of the Chromo domain of human chromodomain helicase-DNA-binding protein 4
Sato, M., Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Chromodomain helicase-DNA-binding protein 4, : 1 : 9 : 38 entities Detail

Interaction partners 1. Chromodomain helicase-DNA-binding protein 4, : 24 interactors Detail

Experiments performed 2 experiments Detail