BMRB: 10275

Solution structures of the PAAD_DAPIN domain of mus musculus interferon-activatable protein 205

Authors

Sato, M., Tochio, N., Koshiba, S., Watanabe, M., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

PAAD DAPIN, UNP residues 8-88

Entity

1. PAAD DAPIN, UNP residues 8-88 (polymer, Thiol state: all free), 94 monomers, 10477.81 Da Detail

GSSGSSGIVL LRGLECINKH YFSLFKSLLA RDLNLERDNQ EQYTTIQIAN MMEEKFPADS GLGKLIEFCE EVPALRKRAE ILKKERSESG PSSG

Formula weight

10477.81 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 93.3 %, Completeness (bb): 90.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.3 % (1023 of 1097)	94.1 % (544 of 578)	91.7 % (386 of 421)	94.9 % (93 of 98)
Backbone	90.0 % (502 of 558)	92.2 % (178 of 193)	87.2 % (239 of 274)	93.4 % (85 of 91)
Sidechain	94.6 % (591 of 625)	93.8 % (361 of 385)	95.7 % (223 of 233)	100.0 % (7 of 7)
Aromatic	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)
Methyl	94.7 % (89 of 94)	93.6 % (44 of 47)	95.7 % (45 of 47)

1. Interferon-activable protein 205

GSSGSSGIVL LRGLECINKH YFSLFKSLLA RDLNLERDNQ EQYTTIQIAN MMEEKFPADS GLGKLIEFCE EVPALRKRAE ILKKERSESG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Interferon-activable protein 205	[U-13C; U-15N]	protein	0.5 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.27 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2YU0, Strand ID: A Detail

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Release date

2009-12-13

Citation

Solution structures of the PAAD_DAPIN domain of mus musculus interferon-activatable protein 205
Sato, M., Tochio, N., Koshiba, S., Watanabe, M., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. PAAD DAPIN, UNP residues 8-88, : 1 : 4 : 31 entities Detail

Interaction partners 1. PAAD DAPIN, UNP residues 8-88, : 11 interactors Detail

Experiments performed 2 experiments Detail