BMRB: 10276

Solution structure of the Tudor domain of Tudor domain containing protein 3 from mouse

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Tudor domain-containing protein 3

Entity

1. Tudor domain-containing protein 3 (polymer, Thiol state: all free), 78 monomers, 8578.308 Da Detail

GSSGSSGKVW KPGDECFALY WEDNKFYRAE VEALHSSGMT AVVKFTDYGN YEEVLLSNIK PVQTEAWVRD PNSGPSSG

Formula weight

8578.308 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.0 %, Completeness: 92.1 %, Completeness (bb): 89.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.1 % (809 of 878)	91.4 % (414 of 453)	93.3 % (320 of 343)	91.5 % (75 of 82)
Backbone	89.3 % (411 of 460)	87.5 % (140 of 160)	90.3 % (204 of 226)	90.5 % (67 of 74)
Sidechain	94.7 % (462 of 488)	93.5 % (274 of 293)	96.3 % (180 of 187)	100.0 % (8 of 8)
Aromatic	94.1 % (96 of 102)	90.2 % (46 of 51)	97.9 % (47 of 48)	100.0 % (3 of 3)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. Tudor domain

GSSGSSGKVW KPGDECFALY WEDNKFYRAE VEALHSSGMT AVVKFTDYGN YEEVLLSNIK PVQTEAWVRD PNSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Tudor domain	[U-13C; U-15N]	protein	1.22 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D9T, Strand ID: A Detail

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Release date

2009-12-13

Citation

Solution structure of the Tudor domain of Tudor domain containing protein 3 from mouse
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Tudor domain-containing protein 3, : 1 : 15 entities Detail

Experiments performed 2 experiments Detail