BMRB: 10277

Solution structure of the Chromo domain of chromobox homolog 2 from human

Authors

Li, H., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Chromobox protein homolog 2 (isoform 2)

Entity

1. Chromobox protein homolog 2 (isoform 2) (polymer, Thiol state: all free), 74 monomers, 8356.272 Da Detail

GSSGSSGEQV FAAECILSKR LRKGKLEYLV KWRGWSSKHN SWEPEENILD PRLLLAFQKK EHEKEVQNSG PSSG

Formula weight

8356.272 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 92.9 %, Completeness (bb): 88.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.9 % (819 of 882)	93.4 % (437 of 468)	93.1 % (311 of 334)	88.7 % (71 of 80)
Backbone	88.6 % (388 of 438)	88.2 % (134 of 152)	89.3 % (192 of 215)	87.3 % (62 of 71)
Sidechain	96.5 % (493 of 511)	95.9 % (303 of 316)	97.3 % (181 of 186)	100.0 % (9 of 9)
Aromatic	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (33 of 33)	100.0 % (3 of 3)
Methyl	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)

1. Chromo domain

GSSGSSGEQV FAAECILSKR LRKGKLEYLV KWRGWSSKHN SWEPEENILD PRLLLAFQKK EHEKEVQNSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	chromo domain	[U-13C; U-15N]	protein	1.11 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.06 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D9U, Strand ID: A Detail

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Release date

2009-12-13

Citation

Solution structure of the Chromo domain of chromobox homolog 2 from human
Li, H., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Chromobox protein homolog 2 (isoform 2), : 1 : 5 : 71 entities Detail

Interaction partners 1. Chromobox protein homolog 2 (isoform 2), : 28 interactors Detail

Experiments performed 2 experiments Detail