BMRB: 10278

Solution structure of the PH domain of Pleckstrin homology domain-containing protein family B member 1 from mouse

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Pleckstrin homology domain-containing protein family B member 1

Entity

1. Pleckstrin homology domain-containing protein family B member 1 (polymer, Thiol state: all free), 130 monomers, 14377.78 Da Detail

GSSGSSGLVR GGWLWRQSSI LRRWKRNWFA LWLDGTLGYY HDETAQDEED RVVIHFNVRD IKVGQECQDV QPPEGRSRDG LLTVNLREGS RLHLCAETRD DAIAWKTALM EANSTPAPAG ATVPSGPSSG

Formula weight

14377.78 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 92.4 %, Completeness (bb): 91.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.4 % (1345 of 1455)	91.4 % (690 of 755)	93.8 % (526 of 561)	92.8 % (129 of 139)
Backbone	91.9 % (706 of 768)	90.7 % (243 of 268)	92.6 % (348 of 376)	92.7 % (115 of 124)
Sidechain	93.2 % (748 of 803)	91.8 % (447 of 487)	95.3 % (287 of 301)	93.3 % (14 of 15)
Aromatic	92.5 % (111 of 120)	93.3 % (56 of 60)	92.6 % (50 of 54)	83.3 % (5 of 6)
Methyl	99.2 % (129 of 130)	98.5 % (64 of 65)	100.0 % (65 of 65)

1. PH domain

GSSGSSGLVR GGWLWRQSSI LRRWKRNWFA LWLDGTLGYY HDETAQDEED RVVIHFNVRD IKVGQECQDV QPPEGRSRDG LLTVNLREGS RLHLCAETRD DAIAWKTALM EANSTPAPAG ATVPSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.12 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.3 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.3 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.25 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D9V, Strand ID: A Detail

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Release date

2008-12-14

Citation

Solution structure of the PH domain of Pleckstrin homology domain-containing protein family B member 1 from mouse
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Pleckstrin homology domain-containing protein family B member 1, : 1 : 4 : 74 entities Detail

Interaction partners 1. Pleckstrin homology domain-containing protein family B member 1, : 6 interactors Detail

Experiments performed 2 experiments Detail