BMRB: 10281

Solution structure of the homeobox domain of the human paired box protein Pax-6

Authors

Ohnishi, S., Kigawa, T., Tochio, N., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Paired box protein Pax6

Entity

1. Paired box protein Pax6 (polymer, Thiol state: not present), 80 monomers, 9406.269 Da Detail

GSSGSSGQRN RTSFTQEQIE ALEKEFERTH YPDVFARERL AAKIDLPEAR IQVWFSNRRA KWRREEKLRN QRRQSGPSSG

Formula weight

9406.269 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.5 %, Completeness: 85.3 %, Completeness (bb): 81.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.3 % (821 of 962)	83.5 % (431 of 516)	89.7 % (321 of 358)	78.4 % (69 of 88)
Backbone	81.6 % (387 of 474)	79.0 % (128 of 162)	84.7 % (199 of 235)	77.9 % (60 of 77)
Sidechain	86.1 % (485 of 563)	83.1 % (294 of 354)	91.9 % (182 of 198)	81.8 % (9 of 11)
Aromatic	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (36 of 36)	100.0 % (2 of 2)
Methyl	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)

1. homeobox domain

GSSGSSGQRN RTSFTQEQIE ALEKEFERTH YPDVFARERL AAKIDLPEAR IQVWFSNRRA KWRREEKLRN QRRQSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.50 mM
2	d-TrisHCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.57 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CUE, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the homeobox domain of the human paired box protein Pax-6
Ohnishi, S., Kigawa, T., Tochio, N., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Paired box protein Pax6, : 1 : 95 entities Detail

Interaction partners 1. Paired box protein Pax6, : 10 interactors Detail

Experiments performed 2 experiments Detail