BMRB: 10282

Solution structure of the homeobox domain of the human hypothetical protein FLJ21616

Authors

Ohnishi, S., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

FLJ21616 protein

Entity

1. FLJ21616 protein (polymer, Thiol state: all free), 95 monomers, 10642.83 Da Detail

GSSGSSGRGS RFTWRKECLA VMESYFNENQ YPDEAKREEI ANACNAVIQK PGKKLSDLER VTSLKVYNWF ANRRKEIKRR ANIAAILESS GPSSG

Formula weight

10642.83 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.6 %, Completeness: 89.4 %, Completeness (bb): 88.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.4 % (984 of 1101)	87.6 % (510 of 582)	91.8 % (382 of 416)	89.3 % (92 of 103)
Backbone	88.7 % (500 of 564)	87.6 % (170 of 194)	89.6 % (249 of 278)	88.0 % (81 of 92)
Sidechain	90.4 % (565 of 625)	87.6 % (340 of 388)	94.7 % (214 of 226)	100.0 % (11 of 11)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (37 of 37)	100.0 % (2 of 2)
Methyl	96.2 % (75 of 78)	94.9 % (37 of 39)	97.4 % (38 of 39)

1. homeobox domain

GSSGSSGRGS RFTWRKECLA VMESYFNENQ YPDEAKREEI ANACNAVIQK PGKKLSDLER VTSLKVYNWF ANRRKEIKRR ANIAAILESS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.40 mM
2	d-TrisHCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2CUF, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the homeobox domain of the human hypothetical protein FLJ21616
Ohnishi, S., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. FLJ21616 protein, : 1 : 3 : 156 entities Detail

Interaction partners 1. FLJ21616 protein, : 5 interactors Detail

Experiments performed 2 experiments Detail