BMRB: 10283

Solution structure of the first homeobox domain of AT-binding transcription factor 1 (ATBF1)

Authors

Ohnishi, S., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Alpha-fetoprotein enhancer binding protein

Entity

1. Alpha-fetoprotein enhancer binding protein (polymer, Thiol state: not present), 70 monomers, 7978.802 Da Detail

GSSGSSGKRP RTRITDDQLR VLRQYFDINN SPSEEQIKEM ADKSGLPQKV IKHWFRNTLF KERQSGPSSG

Formula weight

7978.802 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.4 %, Completeness: 89.4 %, Completeness (bb): 89.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.4 % (745 of 833)	86.3 % (385 of 446)	94.2 % (294 of 312)	88.0 % (66 of 75)
Backbone	89.6 % (369 of 412)	87.3 % (124 of 142)	92.2 % (188 of 204)	86.4 % (57 of 66)
Sidechain	89.9 % (436 of 485)	85.9 % (261 of 304)	96.5 % (166 of 172)	100.0 % (9 of 9)
Aromatic	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (26 of 26)	100.0 % (1 of 1)
Methyl	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)

1. The first homeobox domain

GSSGSSGKRP RTRITDDQLR VLRQYFDINN SPSEEQIKEM ADKSGLPQKV IKHWFRNTLF KERQSGPSSG

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	The first homeobox domain	[U-13C; U-15N]	protein	1.2 mM
2	d-TrisHCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.35 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DA1, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the first homeobox domain of AT-binding transcription factor 1 (ATBF1)
Ohnishi, S., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Alpha-fetoprotein enhancer binding protein, : 1 : 2 : 218 entities Detail

Interaction partners 1. Alpha-fetoprotein enhancer binding protein, : 2 interactors Detail

Experiments performed 2 experiments Detail