BMRB: 10284

Solution structure of the second homeobox domain of AT-binding transcription factor 1 (ATBF1)

Authors

Ohnishi, S., Kigawa, T., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Alpha-fetoprotein enhancer binding protein

Entity

1. Alpha-fetoprotein enhancer binding protein (polymer, Thiol state: not present), 70 monomers, 7898.606 Da Detail

GSSGSSGRSS RTRFTDYQLR VLQDFFDANA YPKDDEFEQL SNLLNLPTRV IVVWFQNARQ KARKSGPSSG

Formula weight

7898.606 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 91.4 %, Completeness (bb): 88.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.4 % (747 of 817)	90.4 % (388 of 429)	92.9 % (289 of 311)	90.9 % (70 of 77)
Backbone	88.9 % (368 of 414)	86.6 % (123 of 142)	90.2 % (185 of 205)	89.6 % (60 of 67)
Sidechain	93.8 % (439 of 468)	92.3 % (265 of 287)	95.9 % (164 of 171)	100.0 % (10 of 10)
Aromatic	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (38 of 38)	100.0 % (1 of 1)
Methyl	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)

1. The second homeobox domain

GSSGSSGRSS RTRFTDYQLR VLQDFFDANA YPKDDEFEQL SNLLNLPTRV IVVWFQNARQ KARKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	The second homeobox domain	[U-13C; U-15N]	protein	1.1 mM
2	d-TrisHCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DA2, Strand ID: A Detail

---

Release date

2009-12-16

Citation

Solution structure of the second homeobox domain of AT-binding transcription factor 1 (ATBF1)
Ohnishi, S., Kigawa, T., Tochio, N., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Alpha-fetoprotein enhancer binding protein, : 1 : 2 : 273 entities Detail

Interaction partners 1. Alpha-fetoprotein enhancer binding protein, : 2 interactors Detail

Experiments performed 2 experiments Detail