BMRB: 10285

Solution structure of the third homeobox domain of AT-binding transcription factor 1 (ATBF1)

Authors

Ohnishi, S., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Alpha-fetoprotein enhancer binding protein

Entity

1. Alpha-fetoprotein enhancer binding protein (polymer, Thiol state: not present), 80 monomers, 8965.955 Da Detail

GSSGSSGGTG GEEPQRDKRL RTTITPEQLE ILYQKYLLDS NPTRKMLDHI AHEVGLKKRV VQVWFQNTRA RERKSGPSSG

Formula weight

8965.955 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 93.9 %, Completeness (bb): 91.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.9 % (872 of 929)	93.5 % (461 of 493)	94.9 % (334 of 352)	91.7 % (77 of 84)
Backbone	91.5 % (432 of 472)	89.7 % (148 of 165)	93.1 % (215 of 231)	90.8 % (69 of 76)
Sidechain	96.2 % (508 of 528)	95.4 % (313 of 328)	97.4 % (187 of 192)	100.0 % (8 of 8)
Aromatic	91.3 % (42 of 46)	91.3 % (21 of 23)	90.9 % (20 of 22)	100.0 % (1 of 1)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. The third homeobox domain

GSSGSSGGTG GEEPQRDKRL RTTITPEQLE ILYQKYLLDS NPTRKMLDHI AHEVGLKKRV VQVWFQNTRA RERKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	The third homeobox domain	[U-13C; U-15N]	protein	1.1 mM
2	d-TrisHCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DA3, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the third homeobox domain of AT-binding transcription factor 1 (ATBF1)
Ohnishi, S., Kigawa, T., Tomizawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Alpha-fetoprotein enhancer binding protein, : 1 : 2 : 81 entities Detail

Interaction partners 1. Alpha-fetoprotein enhancer binding protein, : 4 interactors Detail

Experiments performed 2 experiments Detail