BMRB: 10287

Solution structure of the second homeobox domain of Zinc fingers and homeoboxes protein 3 (Triple homeobox 1 protein)

Authors

Ohnishi, S., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Zinc fingers and homeoboxes protein 3

Entity

1. Zinc fingers and homeoboxes protein 3 (polymer, Thiol state: not present), 75 monomers, 8456.219 Da Detail

GSSGSSGPTK YKERAPEQLR ALESSFAQNP LPLDEELDRL RSETKMTRRE IDSWFSERRK KVNAEETKKS GPSSG

Formula weight

8456.219 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 93.0 %, Completeness (bb): 90.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.0 % (806 of 867)	92.5 % (430 of 465)	94.2 % (308 of 327)	90.7 % (68 of 75)
Backbone	90.7 % (399 of 440)	88.7 % (133 of 150)	92.3 % (203 of 220)	90.0 % (63 of 70)
Sidechain	95.2 % (473 of 497)	94.3 % (297 of 315)	96.6 % (171 of 177)	100.0 % (5 of 5)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (19 of 19)	100.0 % (1 of 1)
Methyl	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)

1. The second homeobox domain

GSSGSSGPTK YKERAPEQLR ALESSFAQNP LPLDEELDRL RSETKMTRRE IDSWFSERRK KVNAEETKKS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	The second homeobox domain	[U-13C; U-15N]	protein	1.2 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.35 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DA5, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the second homeobox domain of Zinc fingers and homeoboxes protein 3 (Triple homeobox 1 protein)
Ohnishi, S., Kigawa, T., Saito, K., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Zinc fingers and homeoboxes protein 3, : 1 : 55 entities Detail

Interaction partners 1. Zinc fingers and homeoboxes protein 3, : 5 interactors Detail

Experiments performed 2 experiments Detail