BMRB: 10288

Solution structure of the homeobox domain of Hepatocyte nuclear factor 1-beta (HNF-1beta)

Authors

Ohnishi, S., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Hepatocyte nuclear factor 1-beta

Entity

1. Hepatocyte nuclear factor 1-beta (polymer, Thiol state: all free), 102 monomers, 11399.42 Da Detail

GSSGSSGRNR FKWGPASQQI LYQAYDRQKN PSKEEREALV EECNRAECLQ RGVSPSKAHG LGSNLVTEVR VYNWFANRRK EEAFRQKLAM DAYSSNSGPS SG

Formula weight

11399.42 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.2 %, Completeness: 90.8 %, Completeness (bb): 88.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.8 % (1059 of 1166)	90.0 % (556 of 618)	92.6 % (403 of 435)	88.5 % (100 of 113)
Backbone	88.2 % (533 of 604)	86.6 % (181 of 209)	89.9 % (267 of 297)	86.7 % (85 of 98)
Sidechain	93.4 % (612 of 655)	91.7 % (375 of 409)	96.1 % (222 of 231)	100.0 % (15 of 15)
Aromatic	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (43 of 43)	100.0 % (2 of 2)
Methyl	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)

1. homeobox domain

GSSGSSGRNR FKWGPASQQI LYQAYDRQKN PSKEEREALV EECNRAECLQ RGVSPSKAHG LGSNLVTEVR VYNWFANRRK EEAFRQKLAM DAYSSNSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.2 mM
2	d-TrisHCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.27 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DA6, Strand ID: A Detail

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Release date

2009-12-16

Citation

A POU-A related region dictates DNA binding specificity of LFB1/HNF1 by orienting the two XL-homeodomains in the dimer
Ohnishi, S., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.
EMBO J. (1992), 11, 4119-4129, PubMed 1356766 , Abstract

Related entities 1. Hepatocyte nuclear factor 1-beta, : 1 : 7 : 30 entities Detail

Interaction partners 1. Hepatocyte nuclear factor 1-beta, : 1 interactors Detail

Experiments performed 2 experiments Detail