BMRB: 10289

Solution structure of the homeobox domain of Zinc finger homeobox protein 1b (Smad interacting protein 1)

Authors

Ohnishi, S., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Zinc finger homeobox protein 1b

Entity

1. Zinc finger homeobox protein 1b (polymer, Thiol state: not present), 71 monomers, 7876.693 Da Detail

GSSGSSGSPI NPYKDHMSVL KAYYAMNMEP NSDELLKISI AVGLPQEFVK EWFEQRKVYQ YSNSRSGPSS G

Formula weight

7876.693 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.5 %, Completeness: 90.3 %, Completeness (bb): 86.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.3 % (742 of 822)	89.4 % (386 of 432)	92.4 % (292 of 316)	86.5 % (64 of 74)
Backbone	86.3 % (359 of 416)	82.5 % (118 of 143)	89.4 % (185 of 207)	84.8 % (56 of 66)
Sidechain	94.1 % (443 of 471)	92.7 % (268 of 289)	96.0 % (167 of 174)	100.0 % (8 of 8)
Aromatic	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (37 of 37)	100.0 % (1 of 1)
Methyl	100.0 % (50 of 50)	100.0 % (25 of 25)	100.0 % (25 of 25)

1. homeobox domain

GSSGSSGSPI NPYKDHMSVL KAYYAMNMEP NSDELLKISI AVGLPQEFVK EWFEQRKVYQ YSNSRSGPSS G

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.2 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DA7, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the homeobox domain of Zinc finger homeobox protein 1b (Smad interacting protein 1)
Ohnishi, S., Kigawa, T., Sato, M., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Zinc finger homeobox protein 1b, : 1 : 4 : 49 entities Detail

Interaction partners 1. Zinc finger homeobox protein 1b, : 2 interactors Detail

Experiments performed 2 experiments Detail