BMRB: 10290

The solution structure of the homeobox domain of human Homeobox protein DLX-5

Authors

Sasagawa, A., Ohnishi, S., Tochio, N., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Homeobox protein DLX-5

Entity

1. Homeobox protein DLX-5 (polymer, Thiol state: not present), 70 monomers, 7728.740 Da Detail

GSSGSSGRKP RTIYSSFQLA ALQRRFQKTQ YLALPERAEL AASLGLTQTQ VKIWFQNKRS KIKKSGPSSG

Formula weight

7728.74 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.6 %, Completeness: 87.2 %, Completeness (bb): 85.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.2 % (728 of 835)	85.6 % (379 of 443)	89.9 % (284 of 316)	85.5 % (65 of 76)
Backbone	85.3 % (353 of 414)	82.5 % (118 of 143)	87.7 % (179 of 204)	83.6 % (56 of 67)
Sidechain	89.5 % (434 of 485)	87.0 % (261 of 300)	93.2 % (164 of 176)	100.0 % (9 of 9)
Aromatic	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (28 of 28)	100.0 % (1 of 1)
Methyl	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (32 of 32)

1. homeobox domain

GSSGSSGRKP RTIYSSFQLA ALQRRFQKTQ YLALPERAEL AASLGLTQTQ VKIWFQNKRS KIKKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.35 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.52 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DJN, Strand ID: A Detail

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Release date

2009-12-16

Citation

The solution structure of the homeobox domain of human Homeobox protein DLX-5
Sasagawa, A., Ohnishi, S., Tochio, N., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Homeobox protein DLX-5, : 1 : 9 : 261 entities Detail

Interaction partners 1. Homeobox protein DLX-5, : 1 interactors Detail

Experiments performed 2 experiments Detail