BMRB: 10291

The solution structure of the homeobox domain of human homeobox protein TGIF2LX

Authors

Tochio, N., Ohnishi, S., Sasagawa, A., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Homeobox protein TGIF2LX

Entity

1. Homeobox protein TGIF2LX (polymer, Thiol state: not present), 83 monomers, 9534.753 Da Detail

GSSGSSGKKR KGNLPAESVK ILRDWMYKHR FKAYPSEEEK QMLSEKTNLS LLQISNWFIN ARRRILPDML QQRRNDPSGP SSG

Formula weight

9534.753 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.1 %, Completeness: 82.5 %, Completeness (bb): 79.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.5 % (830 of 1006)	80.6 % (435 of 540)	85.9 % (324 of 377)	79.8 % (71 of 89)
Backbone	79.9 % (390 of 488)	76.6 % (128 of 167)	82.7 % (201 of 243)	78.2 % (61 of 78)
Sidechain	84.0 % (500 of 595)	81.2 % (303 of 373)	89.6 % (189 of 211)	72.7 % (8 of 11)
Aromatic	100.0 % (64 of 64)	100.0 % (32 of 32)	100.0 % (30 of 30)	100.0 % (2 of 2)
Methyl	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)

1. homeobox domain

GSSGSSGKKR KGNLPAESVK ILRDWMYKHR FKAYPSEEEK QMLSEKTNLS LLQISNWFIN ARRRILPDML QQRRNDPSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.1 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.34 ppm, Outliers: 7 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMN, Strand ID: A Detail

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Release date

2009-12-16

Citation

The solution structure of the homeobox domain of human homeobox protein TGIF2LX
Tochio, N., Ohnishi, S., Sasagawa, A., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Homeobox protein TGIF2LX, : 1 : 5 : 75 entities Detail

Interaction partners 1. Homeobox protein TGIF2LX, : 1 interactors Detail

Experiments performed 2 experiments Detail