BMRB: 10292

Solution structure of the third homeobox domain of Zinc fingers and homeoboxes protein 2

Authors

Ohnishi, S., Sasagawa, A., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Zinc fingers and homeoboxes protein 2

Entity

1. Zinc fingers and homeoboxes protein 2 (polymer, Thiol state: not present), 89 monomers, 9860.849 Da Detail

GSSGSSGAYP DFAPQKFKEK TQGQVKILED SFLKSSFPTQ AELDRLRVET KLSRREIDSW FSERRKLRDS MEQAVLDSMG SGKSGPSSG

Formula weight

9860.849 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 87.2 %, Completeness (bb): 90.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.2 % (895 of 1026)	82.4 % (448 of 544)	93.6 % (366 of 391)	89.0 % (81 of 91)
Backbone	90.3 % (475 of 526)	85.2 % (155 of 182)	93.8 % (243 of 259)	90.6 % (77 of 85)
Sidechain	85.5 % (497 of 581)	80.9 % (293 of 362)	93.9 % (200 of 213)	66.7 % (4 of 6)
Aromatic	88.6 % (62 of 70)	88.6 % (31 of 35)	88.2 % (30 of 34)	100.0 % (1 of 1)
Methyl	96.8 % (60 of 62)	93.5 % (29 of 31)	100.0 % (31 of 31)

1. homeobox domain

GSSGSSGAYP DFAPQKFKEK TQGQVKILED SFLKSSFPTQ AELDRLRVET KLSRREIDSW FSERRKLRDS MEQAVLDSMG SGKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.2 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.32 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMP, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the third homeobox domain of Zinc fingers and homeoboxes protein 2
Ohnishi, S., Sasagawa, A., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Zinc fingers and homeoboxes protein 2, : 1 : 3 : 30 entities Detail

Interaction partners 1. Zinc fingers and homeoboxes protein 2, : 32 interactors Detail

Experiments performed 2 experiments Detail