BMRB: 10293

Solution structure of the homeobox domain of LIM/homeobox protein Lhx9

Authors

Ohnishi, S., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

LIM/homeobox protein Lhx9

Entity

1. LIM/homeobox protein Lhx9 (polymer, Thiol state: not present), 80 monomers, 9000.137 Da Detail

GSSGSSGKRM RTSFKHHQLR TMKSYFAINH NPDAKDLKQL AQKTGLTKRV LQVWFQNARA KFRRNLLRQE NGGVSGPSSG

Formula weight

9000.137 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.7 %, Completeness: 83.7 %, Completeness (bb): 83.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.7 % (797 of 952)	81.6 % (413 of 506)	86.5 % (308 of 356)	84.4 % (76 of 90)
Backbone	83.4 % (397 of 476)	80.7 % (134 of 166)	85.8 % (199 of 232)	82.1 % (64 of 78)
Sidechain	84.7 % (464 of 548)	82.1 % (279 of 340)	88.3 % (173 of 196)	100.0 % (12 of 12)
Aromatic	91.7 % (66 of 72)	91.7 % (33 of 36)	91.4 % (32 of 35)	100.0 % (1 of 1)
Methyl	96.8 % (60 of 62)	93.5 % (29 of 31)	100.0 % (31 of 31)

1. homeobox domain

GSSGSSGKRM RTSFKHHQLR TMKSYFAINH NPDAKDLKQL AQKTGLTKRV LQVWFQNARA KFRRNLLRQE NGGVSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.2 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.25 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMQ, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the homeobox domain of LIM/homeobox protein Lhx9
Ohnishi, S., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. LIM/homeobox protein Lhx9, : 1 : 15 : 86 entities Detail

Interaction partners 1. LIM/homeobox protein Lhx9, : 2 interactors Detail

Experiments performed 2 experiments Detail