BMRB: 10294

Solution structure of the homeobox domain of Homeobox protein OTX2

Authors

Ohnishi, S., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Homeobox protein OTX2

Entity

1. Homeobox protein OTX2 (polymer, Thiol state: all free), 80 monomers, 9100.075 Da Detail

GSSGSSGRRE RTTFTRAQLD VLEALFAKTR YPDIFMREEV ALKINLPESR VQVWFKNRRA KCRQQQQQQQ NGGQSGPSSG

Formula weight

9100.075 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.5 %, Completeness: 78.2 %, Completeness (bb): 78.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.2 % (740 of 946)	74.3 % (375 of 505)	84.6 % (296 of 350)	75.8 % (69 of 91)
Backbone	78.7 % (373 of 474)	75.0 % (123 of 164)	81.5 % (190 of 233)	77.9 % (60 of 77)
Sidechain	78.5 % (428 of 545)	73.9 % (252 of 341)	87.9 % (167 of 190)	64.3 % (9 of 14)
Aromatic	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (29 of 29)	100.0 % (1 of 1)
Methyl	96.8 % (60 of 62)	96.8 % (30 of 31)	96.8 % (30 of 31)

1. homeobox domain

GSSGSSGRRE RTTFTRAQLD VLEALFAKTR YPDIFMREEV ALKINLPESR VQVWFKNRRA KCRQQQQQQQ NGGQSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.0 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.28 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DMS, Strand ID: A Detail

---

Release date

2009-12-16

Citation

Solution structure of the homeobox domain of Homeobox protein OTX2
Ohnishi, S., Saito, K., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Homeobox protein OTX2, : 1 : 7 : 107 entities Detail

Interaction partners 1. Homeobox protein OTX2, : 2 interactors Detail

Experiments performed 2 experiments Detail