BMRB: 10295

Solution structure of the homeobox domain of Homeobox protein BarH-like 1

Authors

Ohnishi, S., Yoneyama, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Homeobox protein BarH-like 1

Entity

1. Homeobox protein BarH-like 1 (polymer, Thiol state: not present), 80 monomers, 8963.101 Da Detail

GSSGSSGGEP GTKAKKGRRS RTVFTELQLM GLEKRFEKQK YLSTPDRIDL AESLGLSQLQ VKTWYQNRRM KWKKSGPSSG

Formula weight

8963.101 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.7 %, Completeness: 86.2 %, Completeness (bb): 85.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.2 % (821 of 952)	83.9 % (427 of 509)	89.7 % (321 of 358)	85.9 % (73 of 85)
Backbone	85.0 % (403 of 474)	83.2 % (139 of 167)	86.5 % (199 of 230)	84.4 % (65 of 77)
Sidechain	87.6 % (480 of 548)	84.2 % (288 of 342)	92.9 % (184 of 198)	100.0 % (8 of 8)
Aromatic	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (28 of 28)	100.0 % (2 of 2)
Methyl	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)

1. homeobox domain

GSSGSSGGEP GTKAKKGRRS RTVFTELQLM GLEKRFEKQK YLSTPDRIDL AESLGLSQLQ VKTWYQNRRM KWKKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.0 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.56 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DMT, Strand ID: A Detail

---

Release date

2009-12-16

Citation

Solution structure of the homeobox domain of Homeobox protein BarH-like 1
Ohnishi, S., Yoneyama, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Homeobox protein BarH-like 1, : 1 : 5 : 109 entities Detail

Interaction partners 1. Homeobox protein BarH-like 1, : 6 interactors Detail

Experiments performed 2 experiments Detail