BMRB: 10296

Solution structure of the homeobox domain of Homeobox protein goosecoid

Authors

Ohnishi, S., Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Homeobox protein goosecoid

Entity

1. Homeobox protein goosecoid (polymer, Thiol state: not present), 70 monomers, 8187.002 Da Detail

GSSGSSGRRH RTIFTDEQLE ALENLFQETK YPDVGTREQL ARKVHLREEK VEVWFKNRRA KWRRSGPSSG

Formula weight

8187.002 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.1 %, Completeness: 85.2 %, Completeness (bb): 83.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.2 % (714 of 838)	83.0 % (370 of 446)	89.0 % (282 of 317)	82.7 % (62 of 75)
Backbone	83.4 % (347 of 416)	79.9 % (115 of 144)	86.8 % (177 of 204)	80.9 % (55 of 68)
Sidechain	87.4 % (425 of 486)	84.4 % (255 of 302)	92.1 % (163 of 177)	100.0 % (7 of 7)
Aromatic	94.3 % (66 of 70)	94.3 % (33 of 35)	93.9 % (31 of 33)	100.0 % (2 of 2)
Methyl	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)

1. homeobox domain

GSSGSSGRRH RTIFTDEQLE ALENLFQETK YPDVGTREQL ARKVHLREEK VEVWFKNRRA KWRRSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.3 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	D2O		solvent	10 %
7	H2O		solvent	90 %

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LACS Plot; CA

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.21 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.3 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DMU, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the homeobox domain of Homeobox protein goosecoid
Ohnishi, S., Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Homeobox protein goosecoid, : 1 : 11 : 262 entities Detail

Interaction partners 1. Homeobox protein goosecoid, : 9 interactors Detail

Experiments performed 2 experiments Detail