BMRB: 10297

Solution structure of the homeobox domain from human NIL-2-A zinc finger protein, transcription factor 8

Authors

Ohnishi, S., Tochio, N., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Assembly

Transcription factor 8

Entity

1. Transcription factor 8 (polymer, Thiol state: not present), 64 monomers, 6820.686 Da Detail

GSSGSSGQPP LKNLLSLLKA YYALNAQPSA EELSKIADSV NLPLDVVKKW FEKMQAGQIS VQSS

Formula weight

6820.686 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

tortion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.2 %, Completeness: 91.7 %, Completeness (bb): 90.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.7 % (676 of 737)	90.6 % (349 of 385)	93.3 % (264 of 283)	91.3 % (63 of 69)
Backbone	90.2 % (339 of 376)	87.5 % (112 of 128)	92.0 % (173 of 188)	90.0 % (54 of 60)
Sidechain	93.6 % (394 of 421)	92.2 % (237 of 257)	95.5 % (148 of 155)	100.0 % (9 of 9)
Aromatic	100.0 % (38 of 38)	100.0 % (19 of 19)	100.0 % (18 of 18)	100.0 % (1 of 1)
Methyl	93.1 % (67 of 72)	94.4 % (34 of 36)	91.7 % (33 of 36)

1. homeobox domain

GSSGSSGQPP LKNLLSLLKA YYALNAQPSA EELSKIADSV NLPLDVVKKW FEKMQAGQIS VQSS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	homeobox domain	[U-13C; U-15N]	protein	1.0 mM
2	d-TrisHCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2E19, Strand ID: A Detail

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Release date

2009-12-16

Citation

Solution structure of the homeobox domain from human NIL-2-A zinc finger protein, transcription factor 8
Ohnishi, S., Tochio, N., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Related entities 1. Transcription factor 8, : 1 : 5 : 25 entities Detail

Interaction partners 1. Transcription factor 8, : 4 interactors Detail

Experiments performed 2 experiments Detail