BMRB: 10299

Solution structures of the fn3 domain of human Tripartite motif protein 9

Authors

Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Tripartite motif protein 9, isoform 2

Entity

1. Tripartite motif protein 9, isoform 2 (polymer, Thiol state: all free), 110 monomers, 11540.62 Da Detail

GSSGSSGPVP ATPILQLEEC CTHNNSATLS WKQPPLSTVP ADGYILELDD GNGGQFREVY VGKETMCTVD GLHFNSTYNA RVKAFNKTGV SPYSKTLVLQ TSEGSGPSSG

Formula weight

11540.62 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 92.5 %, Completeness (bb): 90.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.5 % (1098 of 1187)	93.5 % (571 of 611)	91.4 % (423 of 463)	92.0 % (104 of 113)
Backbone	90.7 % (584 of 644)	92.4 % (208 of 225)	89.0 % (282 of 317)	92.2 % (94 of 102)
Sidechain	94.7 % (606 of 640)	94.0 % (363 of 386)	95.9 % (233 of 243)	90.9 % (10 of 11)
Aromatic	87.8 % (72 of 82)	87.8 % (36 of 41)	87.5 % (35 of 40)	100.0 % (1 of 1)
Methyl	99.1 % (105 of 106)	98.1 % (52 of 53)	100.0 % (53 of 53)

1. Fibronectin type III domain

GSSGSSGPVP ATPILQLEEC CTHNNSATLS WKQPPLSTVP ADGYILELDD GNGGQFREVY VGKETMCTVD GLHFNSTYNA RVKAFNKTGV SPYSKTLVLQ TSEGSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.53 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DB8, Strand ID: A Detail

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Release date

2010-01-10

Citation

Solution structures of the fn3 domain of human Tripartite motif protein 9
Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Tripartite motif protein 9, isoform 2, : 1 : 118 entities Detail

Interaction partners 1. Tripartite motif protein 9, isoform 2, : 30 interactors Detail

Experiments performed 2 experiments Detail