BMRB: 10300

Solution structures of the fn3 domain of human Proto-oncogene tyrosine-protein kinase MER precursor

Authors

Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Proto-oncogene tyrosine-protein kinase MER precursor

Entity

1. Proto-oncogene tyrosine-protein kinase MER precursor (polymer, Thiol state: all free), 124 monomers, 12999.38 Da Detail

GSSGSSGWIL ASTTEGAPSV APLNVTVFLN ESSDNVDIRW MKPPTKQQDG ELVGYRISHV WQSAGISKEL LEEVGQNGSR ARISVQVHNA TCTVRIAAVT RGGVGPFSDP VKIFIPAHSG PSSG

Formula weight

12999.38 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 93.1 %, Completeness (bb): 91.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.1 % (1256 of 1349)	92.7 % (643 of 694)	94.3 % (496 of 526)	90.7 % (117 of 129)
Backbone	91.6 % (667 of 728)	91.3 % (232 of 254)	92.2 % (330 of 358)	90.5 % (105 of 116)
Sidechain	94.7 % (692 of 731)	93.4 % (411 of 440)	96.8 % (269 of 278)	92.3 % (12 of 13)
Aromatic	95.3 % (82 of 86)	95.3 % (41 of 43)	95.0 % (38 of 40)	100.0 % (3 of 3)
Methyl	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (70 of 70)

1. Fibronectin type III domain

GSSGSSGWIL ASTTEGAPSV APLNVTVFLN ESSDNVDIRW MKPPTKQQDG ELVGYRISHV WQSAGISKEL LEEVGQNGSR ARISVQVHNA TCTVRIAAVT RGGVGPFSDP VKIFIPAHSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	fn3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.01 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.01 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.46 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DBJ, Strand ID: A Detail

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Release date

2010-01-10

Citation

Solution structures of the fn3 domain of human Proto-oncogene tyrosine-protein kinase MER precursor
Sato, M., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Proto-oncogene tyrosine-protein kinase MER precursor, : 1 : 2 : 227 entities Detail

Interaction partners 1. Proto-oncogene tyrosine-protein kinase MER precursor, : 4 interactors Detail

Experiments performed 2 experiments Detail