BMRB: 10301

Solution structures of the SH3 domain of human Crk-like protein

Authors

Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Crk-like protein

Entity

1. Crk-like protein (polymer, Thiol state: all free), 88 monomers, 9328.298 Da Detail

GSSGSSGPLP STQNGPVFAK AIQKRVPCAY DKTALALEVG DIVKVTRMNI NGQWEGEVNG RKGLFPFTHV KIFDPQNPDE NESGPSSG

Formula weight

9328.298 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.4 %, Completeness: 85.0 %, Completeness (bb): 82.2 % Detail

Download

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.0 % (836 of 983)	84.5 % (435 of 515)	87.5 % (330 of 377)	78.0 % (71 of 91)
Backbone	82.2 % (421 of 512)	82.1 % (147 of 179)	83.0 % (210 of 253)	80.0 % (64 of 80)
Sidechain	88.1 % (483 of 548)	85.7 % (288 of 336)	93.5 % (188 of 201)	63.6 % (7 of 11)
Aromatic	93.8 % (60 of 64)	93.8 % (30 of 32)	93.5 % (29 of 31)	100.0 % (1 of 1)
Methyl	100.0 % (78 of 78)	100.0 % (39 of 39)	100.0 % (39 of 39)

1. SH3 domain

GSSGSSGPLP STQNGPVFAK AIQKRVPCAY DKTALALEVG DIVKVTRMNI NGQWEGEVNG RKGLFPFTHV KIFDPQNPDE NESGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.44 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DBK, Strand ID: A Detail

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Release date

2010-01-10

Citation

Solution structures of the SH3 domain of human Crk-like protein
Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Crk-like protein, : 1 : 81 entities Detail

Interaction partners 1. Crk-like protein, : 52 interactors Detail

Experiments performed 2 experiments Detail