BMRB: 10302

Solution structures of the SH3 domain of human SH3-containing GRB2-like protein 2

Authors

Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

SH3-containing GRB2-like protein 2

Entity

1. SH3-containing GRB2-like protein 2 (polymer, Thiol state: all free), 73 monomers, 7875.532 Da Detail

GSSGSSGPCC RALYDFEPEN EGELGFKEGD IITLTNQIDE NWYEGMLHGH SGFFPINYVE ILVALPHSGP SSG

Formula weight

7875.532 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 92.7 %, Completeness (bb): 90.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.7 % (746 of 805)	92.3 % (383 of 415)	93.7 % (296 of 316)	90.5 % (67 of 74)
Backbone	90.4 % (387 of 428)	88.8 % (135 of 152)	91.8 % (191 of 208)	89.7 % (61 of 68)
Sidechain	95.2 % (418 of 439)	94.3 % (248 of 263)	96.5 % (164 of 170)	100.0 % (6 of 6)
Aromatic	93.2 % (82 of 88)	93.2 % (41 of 44)	93.0 % (40 of 43)	100.0 % (1 of 1)
Methyl	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)

1. SH3 domain

GSSGSSGPCC RALYDFEPEN EGELGFKEGD IITLTNQIDE NWYEGMLHGH SGFFPINYVE ILVALPHSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SH3 domain	[U-13C; U-15N]	protein	1 mM
2	d-Tris HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.43 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.43 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.57 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DBM, Strand ID: A Detail

---

Release date

2010-01-10

Citation

Solution structures of the SH3 domain of human SH3-containing GRB2-like protein 2
Sato, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. SH3-containing GRB2-like protein 2, : 1 : 8 : 142 entities Detail

Interaction partners 1. SH3-containing GRB2-like protein 2, : 67 interactors Detail

Experiments performed 2 experiments Detail