Solution structure of the C2H2 type zinc finger (region 752-784) of human Zinc finger protein 28 homolog
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS18:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS31:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS35:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.0 % (413 of 480) | 87.0 % (220 of 253) | 87.9 % (160 of 182) | 73.3 % (33 of 45) |
Backbone | 80.0 % (216 of 270) | 79.4 % (77 of 97) | 83.1 % (108 of 130) | 72.1 % (31 of 43) |
Sidechain | 92.7 % (230 of 248) | 91.7 % (143 of 156) | 94.4 % (85 of 90) | 100.0 % (2 of 2) |
Aromatic | 100.0 % (30 of 30) | 100.0 % (15 of 15) | 100.0 % (15 of 15) | |
Methyl | 100.0 % (20 of 20) | 100.0 % (10 of 10) | 100.0 % (10 of 10) |
1. zf-C2H2, UNP residues 752-784
GSSGSSGTGE KPYECSVCGK AFSHRQSLSV HQRIHSGKKP SGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-C2H2, UNP residues 752-784 | [U-13C; U-15N] | protein | 1.0 (±0.1) mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | ZnCl2 | salt | 0.05 mM | |
5 | IDA | salt | 1 mM | |
6 | d-DTT | salt | 1 mM | |
7 | NaN3 | salt | 0.02 % | |
8 | H2O | solvent | 90 % | |
9 | D2O | solvent | 10 % |