BMRB: 10311

Solution structure of the PH domain of PEPP-3 from human

Authors

Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Pleckstrin homology domain-containing protein family A member 6

Entity

1. Pleckstrin homology domain-containing protein family A member 6 (polymer, Thiol state: all free), 117 monomers, 12902.26 Da Detail

GSSGSSGNAP VTKAGWLFKQ ASSGVKQWNK RWFVLVDRCL FYYKDEKEES ILGSIPLLSF RVAAVQPSDN ISRKHTFKAE HAGVRTYFFS AESPEEQEAW IQAMGEAARV QSGPSSG

Formula weight

12902.26 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 96.4 %, Completeness (bb): 95.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.4 % (1304 of 1352)	96.0 % (675 of 703)	97.1 % (509 of 524)	96.0 % (120 of 125)
Backbone	95.4 % (660 of 692)	95.0 % (227 of 239)	95.6 % (326 of 341)	95.5 % (107 of 112)
Sidechain	97.5 % (748 of 767)	96.6 % (448 of 464)	99.0 % (287 of 290)	100.0 % (13 of 13)
Aromatic	100.0 % (150 of 150)	100.0 % (75 of 75)	100.0 % (71 of 71)	100.0 % (4 of 4)
Methyl	98.0 % (100 of 102)	96.1 % (49 of 51)	100.0 % (51 of 51)

1. PH domain

GSSGSSGNAP VTKAGWLFKQ ASSGVKQWNK RWFVLVDRCL FYYKDEKEES ILGSIPLLSF RVAAVQPSDN ISRKHTFKAE HAGVRTYFFS AESPEEQEAW IQAMGEAARV QSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% H2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.14 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.26 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.26 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D9Y, Strand ID: A Detail

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Release date

2010-03-10

Citation

Solution structure of the PH domain of PEPP-3 from human
Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Pleckstrin homology domain-containing protein family A member 6, : 1 : 254 entities Detail

Interaction partners 1. Pleckstrin homology domain-containing protein family A member 6, : 3 interactors Detail

Experiments performed 2 experiments Detail