BMRB: 10312

Solution structure of the PH domain of Protein kinase C, nu type from human

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Solution structure of the PH domain of Protein kinase C, nu type from human

Entity

1. Solution structure of the PH domain of Protein kinase C, nu type from human (polymer, Thiol state: all free), 129 monomers, 14319.84 Da Detail

GSSGSSGMVK EGWMVHYTSR DNLRKRHYWR LDSKCLTLFQ NESGSKYYKE IPLSEILRIS SPRDFTNISQ GSNPHCFEII TDTMVYFVGE NNGDSSHNPV LAATGVGLDV AQSWEKAIRQ ALMSGPSSG

Formula weight

14319.84 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 95.0 %, Completeness (bb): 93.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.0 % (1394 of 1467)	94.5 % (720 of 762)	95.8 % (543 of 567)	94.9 % (131 of 138)
Backbone	93.7 % (716 of 764)	92.8 % (246 of 265)	94.1 % (353 of 375)	94.4 % (117 of 124)
Sidechain	96.3 % (790 of 820)	95.4 % (474 of 497)	97.7 % (302 of 309)	100.0 % (14 of 14)
Aromatic	98.5 % (130 of 132)	98.5 % (65 of 66)	98.4 % (62 of 63)	100.0 % (3 of 3)
Methyl	97.4 % (111 of 114)	96.5 % (55 of 57)	98.2 % (56 of 57)

1. PH domain

GSSGSSGMVK EGWMVHYTSR DNLRKRHYWR LDSKCLTLFQ NESGSKYYKE IPLSEILRIS SPRDFTNISQ GSNPHCFEII TDTMVYFVGE NNGDSSHNPV LAATGVGLDV AQSWEKAIRQ ALMSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	0.42 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.44 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D9Z, Strand ID: A Detail

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Release date

2010-03-10

Citation

Solution structure of the PH domain of Protein kinase C, nu type from human
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Solution structure of the PH domain of Protein kinase C, nu type from human, : 1 : 2 : 29 entities Detail

Interaction partners 1. Solution structure of the PH domain of Protein kinase C, nu type from human, : 12 interactors Detail

Experiments performed 2 experiments Detail