BMRB: 10313

Solution structure of the PH domain of PIP2-dependent ARF1 GTPase-activating protein from human

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S., RIKEN, S.

Assembly

130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein

Entity

1. 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein (polymer, Thiol state: all free), 114 monomers, 12610.00 Da Detail

GSSGSSGYGS EKKGYLLKKS DGIRKVWQRR KCSVKNGILT ISHATSNRQP AKLNLLTCQV KPNAEDKKSF DLISHNRTYH FQAEDEQDYV AWISVLTNSK EEALTMAFSG PSSG

Formula weight

12610.0 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 95.6 %, Completeness (bb): 94.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.6 % (1258 of 1316)	95.2 % (656 of 689)	96.8 % (487 of 503)	92.7 % (115 of 124)
Backbone	94.0 % (637 of 678)	92.3 % (216 of 234)	95.8 % (319 of 333)	91.9 % (102 of 111)
Sidechain	97.3 % (723 of 743)	96.7 % (440 of 455)	98.2 % (270 of 275)	100.0 % (13 of 13)
Aromatic	95.9 % (94 of 98)	95.9 % (47 of 49)	95.7 % (45 of 47)	100.0 % (2 of 2)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. PH domain

GSSGSSGYGS EKKGYLLKKS DGIRKVWQRR KCSVKNGILT ISHATSNRQP AKLNLLTCQV KPNAEDKKSF DLISHNRTYH FQAEDEQDYV AWISVLTNSK EEALTMAFSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.18 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DA0, Strand ID: A Detail

---

Release date

2010-03-10

Citation

Solution structure of the PH domain of PIP2-dependent ARF1 GTPase-activating protein from human
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, : 1 : 122 entities Detail

Interaction partners 1. 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, : 2 interactors Detail

Experiments performed 2 experiments Detail