BMRB: 10314

Solution structure of the PH domain of Evectin-2 from mouse

Authors

Li, H., Yoneyama, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Pleckstrin homology domain-containing family B member 2

Entity

1. Pleckstrin homology domain-containing family B member 2 (polymer, Thiol state: all free), 120 monomers, 13592.14 Da Detail

GSSGSSGFVK SGWLLRQSTI LKRWKKNWFD LWSDGHLIYY DDQTRQSIED KVHMPVDCIN IRTGHECRDI QPPDGKPRDC LLQIVCRDGK TISLCAESTD DCLAWKFTLQ DSRTSGPSSG

Formula weight

13592.14 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 95.4 %, Completeness (bb): 93.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.4 % (1323 of 1387)	94.9 % (688 of 725)	96.6 % (516 of 534)	93.0 % (119 of 128)
Backbone	93.7 % (665 of 710)	92.7 % (227 of 245)	94.9 % (332 of 350)	92.2 % (106 of 115)
Sidechain	97.2 % (765 of 787)	96.0 % (461 of 480)	99.0 % (291 of 294)	100.0 % (13 of 13)
Aromatic	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (54 of 54)	100.0 % (5 of 5)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. PH domain

GSSGSSGFVK SGWLLRQSTI LKRWKKNWFD LWSDGHLIYY DDQTRQSIED KVHMPVDCIN IRTGHECRDI QPPDGKPRDC LLQIVCRDGK TISLCAESTD DCLAWKFTLQ DSRTSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.24 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.37 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.37 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DHI, Strand ID: A Detail

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Release date

2010-03-10

Citation

Solution structure of the PH domain of Evectin-2 from mouse
Li, H., Yoneyama, M., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Pleckstrin homology domain-containing family B member 2, : 1 : 149 entities Detail

Interaction partners 1. Pleckstrin homology domain-containing family B member 2, : 14 interactors Detail

Experiments performed 2 experiments Detail