BMRB: 10315

Solution structure of the PH domain of Rho GTPase activating protein 21 from human

Authors

Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Rho GTPase activating protein 21

Entity

1. Rho GTPase activating protein 21 (polymer, Thiol state: all free), 125 monomers, 14017.53 Da Detail

GSSGSSGDAA KEGWLHFRPL VTDKGKRVGG SIRPWKQMYV VLRGHSLYLY KDKREQTTPS EEEQPISVNA CLIDISYSET KRKNVFRLTT SDCECLFQAE DRDDMLAWIK TIQESSNLNS GPSSG

Formula weight

14017.53 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 93.9 %, Completeness (bb): 91.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.9 % (1350 of 1438)	93.4 % (703 of 753)	95.5 % (528 of 553)	90.2 % (119 of 132)
Backbone	91.6 % (678 of 740)	90.2 % (230 of 255)	93.4 % (341 of 365)	89.2 % (107 of 120)
Sidechain	96.3 % (783 of 813)	95.0 % (473 of 498)	98.3 % (298 of 303)	100.0 % (12 of 12)
Aromatic	99.1 % (105 of 106)	100.0 % (53 of 53)	98.0 % (49 of 50)	100.0 % (3 of 3)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. PH domain

GSSGSSGDAA KEGWLHFRPL VTDKGKRVGG SIRPWKQMYV VLRGHSLYLY KDKREQTTPS EEEQPISVNA CLIDISYSET KRKNVFRLTT SDCECLFQAE DRDDMLAWIK TIQESSNLNS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.01 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.19 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DHJ, Strand ID: A Detail

---

Release date

2010-03-10

Citation

Solution structure of the PH domain of Rho GTPase activating protein 21 from human
Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Rho GTPase activating protein 21, : 1 : 3 : 159 entities Detail

Interaction partners 1. Rho GTPase activating protein 21, : 48 interactors Detail

Experiments performed 2 experiments Detail