BMRB: 10316

Solution structure of the PH domain of TBC1 domain family member 2 protein from human

Authors

Li, H., Tochio, N., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Assembly

TBC1 domain family member 2

Entity

1. TBC1 domain family member 2 (polymer, Thiol state: all free), 119 monomers, 13290.92 Da Detail

GSSGSSGKKL CGYLSKFGGK GPIRGWKSRW FFYDERKCQL YYSRTAQDAN PLDSIDLSSA VFDCKADAEE GIFEIKTPSR VITLKAATKQ AMLYWLQQLQ MKRWEFHNSP PAPSGPSSG

Formula weight

13290.92 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.4 %, Completeness: 92.4 %, Completeness (bb): 90.0 % Detail

Download

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.4 % (1296 of 1403)	91.3 % (673 of 737)	94.5 % (512 of 542)	89.5 % (111 of 124)
Backbone	90.0 % (630 of 700)	88.0 % (213 of 242)	91.9 % (318 of 346)	88.4 % (99 of 112)
Sidechain	94.8 % (769 of 811)	92.9 % (460 of 495)	97.7 % (297 of 304)	100.0 % (12 of 12)
Aromatic	98.7 % (150 of 152)	100.0 % (76 of 76)	97.2 % (70 of 72)	100.0 % (4 of 4)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. PH domain

GSSGSSGKKL CGYLSKFGGK GPIRGWKSRW FFYDERKCQL YYSRTAQDAN PLDSIDLSSA VFDCKADAEE GIFEIKTPSR VITLKAATKQ AMLYWLQQLQ MKRWEFHNSP PAPSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.31 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.07 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.07 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.14 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DHK, Strand ID: A Detail

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Release date

2010-03-10

Citation

Solution structure of the PH domain of TBC1 domain family member 2 protein from human
Li, H., Tochio, N., Koshiba, S., Harada, T., Watanabe, S., Kigawa, T., Yokoyama, S.

Related entities 1. TBC1 domain family member 2, : 1 : 2 : 91 entities Detail

Interaction partners 1. TBC1 domain family member 2, : 8 interactors Detail

Experiments performed 2 experiments Detail