BMRB: 10317

Solution structure of the PH domain of pleckstrin homology domain-containing protein family A member 5 from human

Authors

Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Pleckstrin homology domain-containing family A member 5

Entity

1. Pleckstrin homology domain-containing family A member 5 (polymer, Thiol state: all free), 128 monomers, 14577.58 Da Detail

GSSGSSGKRS NSIKRNPNAP VVRRGWLYKQ DSTGMKLWKK RWFVLSDLCL FYYRDEKEEG ILGSILLPSF QIALLTSEDH INRKYAFKAA HPNMRTYYFC TDTGKEMELW MKAMLDAALV QTSGPSSG

Formula weight

14577.58 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 94.7 %, Completeness (bb): 92.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.7 % (1446 of 1527)	94.9 % (758 of 799)	95.3 % (565 of 593)	91.1 % (123 of 135)
Backbone	92.2 % (699 of 758)	92.0 % (240 of 261)	93.0 % (348 of 374)	90.2 % (111 of 123)
Sidechain	96.8 % (859 of 887)	96.3 % (518 of 538)	97.6 % (329 of 337)	100.0 % (12 of 12)
Aromatic	98.7 % (152 of 154)	100.0 % (77 of 77)	97.3 % (71 of 73)	100.0 % (4 of 4)
Methyl	98.3 % (114 of 116)	96.6 % (56 of 58)	100.0 % (58 of 58)

1. PH domain

GSSGSSGKRS NSIKRNPNAP VVRRGWLYKQ DSTGMKLWKK RWFVLSDLCL FYYRDEKEEG ILGSILLPSF QIALLTSEDH INRKYAFKAA HPNMRTYYFC TDTGKEMELW MKAMLDAALV QTSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]		1.14 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.07 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.07 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.36 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DKP, Strand ID: A Detail

---

Release date

2010-03-10

Citation

Solution structure of the PH domain of pleckstrin homology domain-containing protein family A member 5 from human"
Li, H., Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Pleckstrin homology domain-containing family A member 5, : 1 : 2 : 110 entities Detail

Interaction partners 1. Pleckstrin homology domain-containing family A member 5, : 52 interactors Detail

Experiments performed 2 experiments Detail