BMRB: 10318

Solution structure of the PTB domain of KIAA1075 protein from human

Authors

Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

KIAA1075 protein

Entity

1. KIAA1075 protein (polymer, Thiol state: all free), 160 monomers, 16955.89 Da Detail

GSSGSSGMST AADLLRQGAA CSVLYLTSVE TESLTGPQAV ARASSAALSC SPRPTPAVVH FKVSAQGITL TDNQRKLFFR RHYPVNSITF SSTDPQDRRW TNPDGTTSKI FGFVAKKPGS PWENVCHLFA ELDPDQPAGA IVTFITKVLL GQRKSGPSSG

Formula weight

16955.89 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 96.5 %, Completeness (bb): 95.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.5 % (1704 of 1765)	96.6 % (879 of 910)	96.7 % (671 of 694)	95.7 % (154 of 161)
Backbone	95.4 % (893 of 936)	95.6 % (307 of 321)	95.3 % (445 of 467)	95.3 % (141 of 148)
Sidechain	98.1 % (957 of 976)	97.1 % (572 of 589)	99.5 % (372 of 374)	100.0 % (13 of 13)
Aromatic	99.2 % (131 of 132)	100.0 % (66 of 66)	98.4 % (63 of 64)	100.0 % (2 of 2)
Methyl	99.4 % (169 of 170)	98.8 % (84 of 85)	100.0 % (85 of 85)

1. PTB domain

GSSGSSGMST AADLLRQGAA CSVLYLTSVE TESLTGPQAV ARASSAALSC SPRPTPAVVH FKVSAQGITL TDNQRKLFFR RHYPVNSITF SSTDPQDRRW TNPDGTTSKI FGFVAKKPGS PWENVCHLFA ELDPDQPAGA IVTFITKVLL GQRKSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PTB domain	[U-13C; U-15N]	protein	0.57 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DKQ, Strand ID: A Detail

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Release date

2010-03-10

Citation

Solution structure of the PTB domain of KIAA1075 protein from human
Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. KIAA1075 protein, : 1 : 1 : 33 entities Detail

Interaction partners 1. KIAA1075 protein, : 35 interactors Detail

Experiments performed 2 experiments Detail