BMRB: 10319

Solution structure of the PH domain of KIAA0640 protein from human

Authors

Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

KIAA0640 protein

Entity

1. KIAA0640 protein (polymer, Thiol state: all free), 115 monomers, 12997.88 Da Detail

GSSGSSGVLK QGYMMKKGHR RKNWTERWFV LKPNIISYYV SEDLKDKKGD ILLDENCCVE SLPDKDGKKC LFLVKCFDKT FEISASDKKK KQEWIQAIHS TIHLLKLGSS GPSSG

Formula weight

12997.88 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 94.3 %, Completeness (bb): 92.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	94.3 % (1307 of 1386)	93.8 % (686 of 731)	95.5 % (510 of 534)	91.7 % (111 of 121)
Backbone	92.4 % (632 of 684)	91.6 % (217 of 237)	93.4 % (313 of 335)	91.1 % (102 of 112)
Sidechain	96.3 % (777 of 807)	94.9 % (469 of 494)	98.4 % (299 of 304)	100.0 % (9 of 9)
Aromatic	96.4 % (108 of 112)	96.4 % (54 of 56)	96.2 % (51 of 53)	100.0 % (3 of 3)
Methyl	99.0 % (101 of 102)	98.0 % (50 of 51)	100.0 % (51 of 51)

1. PH domain

GSSGSSGVLK QGYMMKKGHR RKNWTERWFV LKPNIISYYV SEDLKDKKGD ILLDENCCVE SLPDKDGKKC LFLVKCFDKT FEISASDKKK KQEWIQAIHS TIHLLKLGSS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	PH domain	[U-13C; U-15N]	protein	1.17 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.31 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DN6, Strand ID: A Detail

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Release date

2010-03-10

Citation

Solution structure of the PH domain of KIAA0640 protein from human
Li, H., Tochio, N., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. KIAA0640 protein, : 1 : 2 : 250 entities Detail

Interaction partners 1. KIAA0640 protein, : 5 interactors Detail

Experiments performed 2 experiments Detail