BMRB: 10321

Solution structure of the 11th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 128 monomers, 13440.58 Da Detail

GSSGSSGHFP ARVKVEPAVD TSRIKVFGPG IEGKDVFREA TTDFTVDSRP LTQVGGDHIK AHIANPSGAS TECFVTDNAD GTYQVEYTPF EKGLHVVEVT YDDVPIPNSP FKVAVTEGCQ PSSGPSSG

Formula weight

13440.58 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.0 %, Completeness: 92.1 %, Completeness (bb): 91.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.1 % (1265 of 1373)	91.2 % (642 of 704)	93.6 % (511 of 546)	91.1 % (112 of 123)
Backbone	91.3 % (681 of 746)	90.0 % (233 of 259)	92.4 % (342 of 370)	90.6 % (106 of 117)
Sidechain	93.3 % (691 of 741)	91.9 % (409 of 445)	95.2 % (276 of 290)	100.0 % (6 of 6)
Aromatic	90.9 % (100 of 110)	90.9 % (50 of 55)	90.9 % (50 of 55)
Methyl	100.0 % (126 of 126)	100.0 % (63 of 63)	100.0 % (63 of 63)

1. Filamin domain

GSSGSSGHFP ARVKVEPAVD TSRIKVFGPG IEGKDVFREA TTDFTVDSRP LTQVGGDHIK AHIANPSGAS TECFVTDNAD GTYQVEYTPF EKGLHVVEVT YDDVPIPNSP FKVAVTEGCQ PSSGPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.22 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DIB, Strand ID: A Detail

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Release date

2010-04-06

Citation

Solution structure of the 11th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 275 entities Detail

Interaction partners 1. Filamin-B, : 62 interactors Detail

Experiments performed 2 experiments Detail