Solution structure of the 15th Filamin domain from human Filamin-B
GSSGSSGTGD ASKCLATGPG IASTVKTGEE VGFVVDAKTA GKGKVTCTVL TPDGTEAEAD VIENEDGTYD IFYTAAKPGT YVIYVRFGGV DIPNSPFTVM ATDGEVTAVE EAPVNACPSG PSSG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.3 % (1015 of 1248) | 80.4 % (505 of 628) | 82.4 % (413 of 501) | 81.5 % (97 of 119) |
Backbone | 79.3 % (577 of 728) | 77.5 % (200 of 258) | 79.7 % (282 of 354) | 81.9 % (95 of 116) |
Sidechain | 83.4 % (522 of 626) | 82.4 % (305 of 370) | 85.0 % (215 of 253) | 66.7 % (2 of 3) |
Aromatic | 100.0 % (72 of 72) | 100.0 % (36 of 36) | 100.0 % (36 of 36) | |
Methyl | 83.6 % (117 of 140) | 82.9 % (58 of 70) | 84.3 % (59 of 70) |
1. Filamin domain
GSSGSSGTGD ASKCLATGPG IASTVKTGEE VGFVVDAKTA GKGKVTCTVL TPDGTEAEAD VIENEDGTYD IFYTAAKPGT YVIYVRFGGV DIPNSPFTVM ATDGEVTAVE EAPVNACPSG PSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Filamin domain | [U-13C; U-15N] | protein | 1.21 mM |
2 | d-Tris-HCl | natural abundance | buffer | 20 mM |
3 | NaCl | natural abundance | salt | 100 mM |
4 | d-DTT | natural abundance | salt | 1 mM |
5 | NaN3 | natural abundance | salt | 0.02 % |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |