BMRB: 10327

Solution structure of the filamin domain from human tripartite motif protein 45

Authors

Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Assembly

Tripartite motif protein 45

Entity

1. Tripartite motif protein 45 (polymer, Thiol state: all free), 113 monomers, 12367.81 Da Detail

GSSGSSGEVD PAKCVLQGED LHRAREKQTA SFTLLCKDAA GEIMGRGGDN VQVAVVPKDK KDSPVRTMVQ DNKDGTYYIS YTPKEPGVYT VWVCIKEQHV QGSPFTVTVR RKH

Formula weight

12367.81 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.9 %, Completeness (bb): 98.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.9 % (1256 of 1270)	98.3 % (651 of 662)	99.8 % (491 of 492)	98.3 % (114 of 116)
Backbone	98.9 % (659 of 666)	98.3 % (227 of 231)	99.7 % (327 of 328)	98.1 % (105 of 107)
Sidechain	99.0 % (699 of 706)	98.4 % (424 of 431)	100.0 % (266 of 266)	100.0 % (9 of 9)
Aromatic	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (37 of 37)	100.0 % (1 of 1)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. Filamin domain

GSSGSSGEVD PAKCVLQGED LHRAREKQTA SFTLLCKDAA GEIMGRGGDN VQVAVVPKDK KDSPVRTMVQ DNKDGTYYIS YTPKEPGVYT VWVCIKEQHV QGSPFTVTVR RKH

Show sample condition

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.15 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.84 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DS4, Strand ID: A Detail

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Release date

2010-04-06

Citation

Solution structure of the filamin domain from human tripartite motif protein 45
Tomizawa, T., Koshiba, S., Watanabe, S., Harada, T., Kigawa, T., Yokoyama, S.

Related entities 1. Tripartite motif protein 45, : 1 : 266 entities Detail

Interaction partners 1. Tripartite motif protein 45, : 1 interactors Detail

Experiments performed 2 experiments Detail