BMRB: 10328

Solution structure of the 14th Filamin domain from human Filamin C

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Filamin-C

Entity

1. Filamin-C (polymer, Thiol state: not present), 115 monomers, 11737.76 Da Detail

GSSGSSGAHD ASKVRASGPG LNASGIPASL PVEFTIDARD AGEGLLTVQI LDPEGKPKKA NIRDNGDGTY TVSYLPDMSG RYTITIKYGG DEIPYSPFRI HALPTGDASS GPSSG

Formula weight

11737.76 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.6 %, Completeness (bb): 97.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.6 % (1176 of 1205)	97.6 % (607 of 622)	97.7 % (463 of 474)	97.2 % (106 of 109)
Backbone	97.8 % (655 of 670)	98.3 % (233 of 237)	97.6 % (320 of 328)	97.1 % (102 of 105)
Sidechain	97.5 % (617 of 633)	97.1 % (374 of 385)	98.0 % (239 of 244)	100.0 % (4 of 4)
Aromatic	89.7 % (61 of 68)	88.2 % (30 of 34)	91.2 % (31 of 34)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. Filamin domain

GSSGSSGAHD ASKVRASGPG LNASGIPASL PVEFTIDARD AGEGLLTVQI LDPEGKPKKA NIRDNGDGTY TVSYLPDMSG RYTITIKYGG DEIPYSPFRI HALPTGDASS GPSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.22 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.55 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D7M, Strand ID: A Detail

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Release date

2010-04-13

Citation

Solution structure of the 14th Filamin domain from human Filamin C
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Filamin-C, : 1 : 5 : 337 entities Detail

Interaction partners 1. Filamin-C, : 51 interactors Detail

Experiments performed 2 experiments Detail