BMRB: 10329

Solution structure of the 16th Filamin domain from human Filamin C

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Filamin-C

Entity

1. Filamin-C (polymer, Thiol state: not present), 93 monomers, 9867.934 Da Detail

GSSGSSGLRP FNLVIPFAVQ KGELTGEVRM PSGKTARPNI TDNKDGTITV RYAPTEKGLH QMGIKYDGNH IPGSPLQFYV DAINSRHSGP SSG

Formula weight

9867.934 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 95.0 %, Completeness (bb): 94.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.0 % (979 of 1031)	93.1 % (502 of 539)	97.7 % (390 of 399)	93.5 % (87 of 93)
Backbone	94.8 % (514 of 542)	93.2 % (178 of 191)	96.6 % (257 of 266)	92.9 % (79 of 85)
Sidechain	95.4 % (543 of 569)	93.1 % (324 of 348)	99.1 % (211 of 213)	100.0 % (8 of 8)
Aromatic	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)
Methyl	96.4 % (81 of 84)	92.9 % (39 of 42)	100.0 % (42 of 42)

1. Filamin domain

GSSGSSGLRP FNLVIPFAVQ KGELTGEVRM PSGKTARPNI TDNKDGTITV RYAPTEKGLH QMGIKYDGNH IPGSPLQFYV DAINSRHSGP SSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.19 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.12 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.74 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D7N, Strand ID: A Detail

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Release date

2010-04-13

Citation

Solution structure of the 16th Filamin domain from human Filamin C
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Filamin-C, : 1 : 3 : 52 entities Detail

Interaction partners 1. Filamin-C, : 53 interactors Detail

Experiments performed 2 experiments Detail