BMRB: 10330

Solution structure of the 17th Filamin domain from human Filamin C

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Filamin-C

Entity

1. Filamin-C (polymer, Thiol state: all free), 111 monomers, 11246.32 Da Detail

GSSGSSGAIN SRHVSAYGPG LSHGMVNKPA TFTIVTKDAG EGGLSLAVEG PSKAEITCKD NKDGTCTVSY LPTAPGDYSI IVRFDDKHIP GSPFTAKITG DDSMRSGPSS G

Formula weight

11246.32 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 95.4 %, Completeness (bb): 95.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.4 % (1098 of 1151)	93.9 % (555 of 591)	97.4 % (442 of 454)	95.3 % (101 of 106)
Backbone	95.1 % (618 of 650)	93.5 % (215 of 230)	96.2 % (305 of 317)	95.1 % (98 of 103)
Sidechain	95.8 % (571 of 596)	94.2 % (340 of 361)	98.3 % (228 of 232)	100.0 % (3 of 3)
Aromatic	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. Filamin domain

GSSGSSGAIN SRHVSAYGPG LSHGMVNKPA TFTIVTKDAG EGGLSLAVEG PSKAEITCKD NKDGTCTVSY LPTAPGDYSI IVRFDDKHIP GSPFTAKITG DDSMRSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.16 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.83 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D7O, Strand ID: A Detail

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Release date

2010-04-13

Citation

Solution structure of the 17th Filamin domain from human Filamin C
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Filamin-C, : 1 : 285 entities Detail

Interaction partners 1. Filamin-C, : 15 interactors Detail

Experiments performed 2 experiments Detail