BMRB: 10331

Solution structure of the 22th Filamin domain from human Filamin C

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Filamin-C

Entity

1. Filamin-C (polymer, Thiol state: all free), 112 monomers, 11655.62 Da Detail

GSSGSSGSDD ARRLTVTSLQ ETGLKVNQPA SFAVQLNGAR GVIDARVHTP SGAVEECYVS ELDSDKHTIR FIPHENGVHS IDVKFNGAHI PGSPFKIRVG EQSQAGSGPS SG

Formula weight

11655.62 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 96.1 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.1 % (1144 of 1191)	95.9 % (590 of 615)	96.7 % (446 of 461)	93.9 % (108 of 115)
Backbone	95.3 % (629 of 660)	95.7 % (222 of 232)	95.7 % (308 of 322)	93.4 % (99 of 106)
Sidechain	97.0 % (610 of 629)	96.1 % (368 of 383)	98.3 % (233 of 237)	100.0 % (9 of 9)
Aromatic	97.1 % (66 of 68)	97.1 % (33 of 34)	97.1 % (33 of 34)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. Filamin domain

GSSGSSGSDD ARRLTVTSLQ ETGLKVNQPA SFAVQLNGAR GVIDARVHTP SGAVEECYVS ELDSDKHTIR FIPHENGVHS IDVKFNGAHI PGSPFKIRVG EQSQAGSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.24 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.49 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D7P, Strand ID: A Detail

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Release date

2010-04-13

Citation

Solution structure of the 22th Filamin domain from human Filamin C
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Filamin-C, : 1 : 132 entities Detail

Interaction partners 1. Filamin-C, : 15 interactors Detail

Experiments performed 2 experiments Detail