BMRB: 10332

Solution structure of the 23th Filamin domain from human Filamin C

Authors

Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Assembly

Filamin-C

Entity

1. Filamin-C (polymer, Thiol state: all free), 111 monomers, 10849.95 Da Detail

GSSGSSGAGD PGLVSAYGPG LEGGTTGVSS EFIVNTLNAG SGALSVTIDG PSKVQLDCRE CPEGHVVTYT PMAPGNYLIA IKYGGPQHIV GSPFKAKVTG PRLSGSGPSS G

Formula weight

10849.95 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 93.5 %, Completeness (bb): 92.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.5 % (1057 of 1131)	92.7 % (537 of 579)	94.6 % (422 of 446)	92.5 % (98 of 106)
Backbone	92.1 % (595 of 646)	91.9 % (215 of 234)	92.3 % (287 of 311)	92.1 % (93 of 101)
Sidechain	94.9 % (545 of 574)	93.3 % (322 of 345)	97.3 % (218 of 224)	100.0 % (5 of 5)
Aromatic	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (30 of 30)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. Filamin domain

GSSGSSGAGD PGLVSAYGPG LEGGTTGVSS EFIVNTLNAG SGALSVTIDG PSKVQLDCRE CPEGHVVTYT PMAPGNYLIA IKYGGPQHIV GSPFKAKVTG PRLSGSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.13 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.14 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.14 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.7 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2D7Q, Strand ID: A Detail

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Release date

2010-04-13

Citation

Solution structure of the 23th Filamin domain from human Filamin C
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.

Related entities 1. Filamin-C, : 1 : 4 : 252 entities Detail

Interaction partners 1. Filamin-C, : 54 interactors Detail

Experiments performed 2 experiments Detail