BMRB: 10333

Solution structure of the filamin domain from human BK158_1 protein

Authors

Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

BK158 1

Entity

1. BK158 1 (polymer, Thiol state: not present), 124 monomers, 13494.02 Da Detail

GSSGSSGETG GERQLSPEKS EIWGPGLKAD VVLPARYFYI QAVDTSGNKF TSSPGEKVFQ VKVSAPEEQF TRVGVQVLDR KDGSFIVRYR MYASYKNLKV EIKFQGQHVA KSPYILKGSG PSSG

Formula weight

13494.02 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 95.0 %, Completeness (bb): 93.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.0 % (1360 of 1432)	94.9 % (712 of 750)	96.2 % (534 of 555)	89.8 % (114 of 127)
Backbone	93.0 % (679 of 730)	93.0 % (238 of 256)	94.4 % (337 of 357)	88.9 % (104 of 117)
Sidechain	96.9 % (786 of 811)	96.0 % (474 of 494)	98.4 % (302 of 307)	100.0 % (10 of 10)
Aromatic	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (61 of 61)	100.0 % (1 of 1)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. Filamin-type immunoglobulin domains

GSSGSSGETG GERQLSPEKS EIWGPGLKAD VVLPARYFYI QAVDTSGNKF TSSPGEKVFQ VKVSAPEEQF TRVGVQVLDR KDGSFIVRYR MYASYKNLKV EIKFQGQHVA KSPYILKGSG PSSG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	1.11 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.12 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.71 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2DI7, Strand ID: A Detail

---

Release date

2010-04-13

Citation

Solution structure of the filamin domain from human BK158_1 protein
Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. BK158 1, : 1 : 2 : 64 entities Detail

Interaction partners 1. BK158 1, : 1 interactors Detail

Experiments performed 2 experiments Detail