BMRB: 10334

Solution structure of the 19th filamin domain from human Filamin-B

Authors

Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Assembly

Filamin-B

Entity

1. Filamin-B (polymer, Thiol state: all free), 111 monomers, 11524.58 Da Detail

GSSGSSGIGD ARRAKVYGRG LSEGRTFEMS DFIVDTRDAG YGGISLAVEG PSKVDIQTED LEDGTCKVSY FPTVPGVYIV STKFADEHVP GSPFTVKISG EGRVKSGPSS G

Formula weight

11524.58 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, restrainted molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 95.9 %, Completeness (bb): 94.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.9 % (1131 of 1179)	95.6 % (580 of 607)	97.0 % (452 of 466)	93.4 % (99 of 106)
Backbone	94.8 % (620 of 654)	94.4 % (221 of 234)	95.9 % (302 of 315)	92.4 % (97 of 105)
Sidechain	97.1 % (600 of 618)	96.2 % (359 of 373)	98.4 % (240 of 244)	100.0 % (1 of 1)
Aromatic	97.7 % (84 of 86)	97.7 % (42 of 43)	97.7 % (42 of 43)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. Filamin domain

GSSGSSGIGD ARRAKVYGRG LSEGRTFEMS DFIVDTRDAG YGGISLAVEG PSKVDIQTED LEDGTCKVSY FPTVPGVYIV STKFADEHVP GSPFTVKISG EGRVKSGPSS G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Filamin domain	[U-13C; U-15N]	protein	0.80 mM
2	d-Tris-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: 0.19 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.19 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.57 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2DI8, Strand ID: A Detail

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Release date

2010-04-13

Citation

Solution structure of the 19th filamin domain from human Filamin-B
Tomizawa, T., Koshiba, S., Inoue, M., Kigawa, T., Yokoyama, S.

Related entities 1. Filamin-B, : 1 : 3 : 336 entities Detail

Interaction partners 1. Filamin-B, : 68 interactors Detail

Experiments performed 2 experiments Detail