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Found 1 Document (1.37 seconds)

BMRB: 10336

1WWY

Solution structure of the DUF1000 domain of a thioredoxin-like protein 1

Authors

Goroncy, A.K., Kigawa, T., Koshiba, S., Kobayashi, N., Tochio, N., Inoue, M., Yokoyama, S.

Assembly

Thioredoxin-like protein 1

Entity

1. Thioredoxin-like protein 1 (polymer, Thiol state: all free), 171 monomers, 19067.96 Da Detail

GSSGSSGGYM DLMPFINKAG CECLNESDEH GFDNCLRKDT TFLESDCDEQ LLITVAFNQP VKLYSMKFQG PDNGQGPKYV KIFINLPRSM DFEEAERSEP TQALELTEDD IKEDGIVPLR YVKFQNVNSV TIFVQSNQGE EETTRISYFT FIGTPVQATN MNDFKSGPSS G

Formula weight

19067.96 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.5 %, Completeness (bb): 99.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	99.5 % (1956 of 1966)	99.3 % (1018 of 1025)	99.9 % (758 of 759)	98.9 % (180 of 182)
Backbone	99.3 % (1001 of 1008)	98.8 % (343 of 347)	99.8 % (498 of 499)	98.8 % (160 of 162)
Sidechain	99.6 % (1111 of 1115)	99.6 % (675 of 678)	99.8 % (416 of 417)	100.0 % (20 of 20)
Aromatic	100.0 % (164 of 164)	100.0 % (82 of 82)	100.0 % (82 of 82)
Methyl	100.0 % (148 of 148)	100.0 % (74 of 74)	100.0 % (74 of 74)

1. DUF1000 domain

GSSGSSGGYM DLMPFINKAG CECLNESDEH GFDNCLRKDT TFLESDCDEQ LLITVAFNQP VKLYSMKFQG PDNGQGPKYV KIFINLPRSM DFEEAERSEP TQALELTEDD IKEDGIVPLR YVKFQNVNSV TIFVQSNQGE EETTRISYFT FIGTPVQATN MNDFKSGPSS G

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.02mM DUF1000 domain (15N, 13C -labeled), 20mM Tris-d11-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	null	indirect	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1WWY, Strand ID: A Detail

Release date

2010-12-08

Citation

Solution structure of the C-terminal DUF1000 domain of the human thioredoxin-like 1 protein
Goroncy, A.K., Koshiba, S., Tochio, N., Tomizawa, T., Inoue, M., Tanaka, A., Sugano, S., Kigawa, T., Yokoyama, S.
Proteins (2010), 78, 2176-2180, PubMed 20455272 , DOI 10.1002/prot.22719 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: O43396 released on 2001-10-18
Title TXNL1_HUMAN Entity Thioredoxin-like protein 1

Related entities 1. Thioredoxin-like protein 1, : 1 : 2 : 35 entities Detail

Interaction partners 1. Thioredoxin-like protein 1, : 10 interactors Detail

More details for 10 interactors identified by 7 citations

Experiments performed 18 experiments Detail

1 3D 15N-separated NOESY

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.02mM DUF1000 domain (15N, 13C -labeled), 20mM Tris-d11-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

2 3D 13C-separated NOESY

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

3 CBCACONH

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

4 CCCONNH

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

5 CCH TOCSY

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

6 HBHACONH

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

7 HCCCONNH

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

8 HCCH COSY

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

9 HCCH TOCSY

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

10 3D HNCA

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

11 3D HNCACB

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

12 3D HNCACO

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

13 3D HNCO

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

14 3D HNCOCA

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

15 C13 HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

16 N15 HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

17 3D 1H-13C NOESY-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

18 3D 1H-15N NOESY-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	DUF1000 domain	[U-13C; U-15N]	protein	1.02 mM
2	Tris-d11-HCl		buffer	20 mM
3	NaCl		salt	100 mM
4	d-DTT		salt	1 mM
5	NaN3		salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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Found 1 Document (1.37 seconds)

BMRB: 10336

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Thioredoxin-like protein 1, : 1 : 2 : 35 entities Detail

Interaction partners 1. Thioredoxin-like protein 1, : 10 interactors Detail

Experiments performed 18 experiments Detail

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