BMRB: 11002

NMR structure of the antimicrobial peptide RP-1 bound to SDS micelles

Authors

Bourbigot, S., Booth, V., Dodd, E., Horwood, C., Welch, W.H., Sharma, S., Waring, A.J., Yeaman, M.R.

Assembly

antimicrobial peptide RP-1

Entity

1. antimicrobial peptide RP-1 (polymer, Thiol state: not present), 18 monomers, 2162.747 Da Detail

ALYKKFKKKL LKSLKRLG

Formula weight

2162.747 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.5 %, Completeness (bb): 78.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	92.5 % (147 of 159)	100.0 % (141 of 141)	33.3 % (6 of 18)
Backbone	78.2 % (43 of 55)	100.0 % (37 of 37)	33.3 % (6 of 18)
Sidechain	100.0 % (104 of 104)	100.0 % (104 of 104)
Aromatic	100.0 % (9 of 9)	100.0 % (9 of 9)
Methyl	100.0 % (11 of 11)	100.0 % (11 of 11)

1. antimicrobial peptide RP-1 subunit 1

ALYKKFKKKL LKSLKRLG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-15N]-Leu		1.5 mM
2	DSS	natural abundance		0.4 mM
3	SDS	[U-99% 2H]		150 mM
4	sodium azide	natural abundance		0.4 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2RLG, Strand ID: A Detail

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Release date

2008-08-24

Citation

Antimicrobial peptide RP-1 structure and interactions with anionic versus zwitterionic micelles
Bourbigot, S., Dodd, E., Horwood, C., Cumby, N., Fardy, L., Welch, W.H., Ramjan, Z., Sharma, S., Waring, A.J., Yeaman, M.R., Booth, V.
Biopolymers (2009), 91, 1-13, PubMed 18712851 , DOI 10.1002/bip.21071 , Abstract

Related entities 1. antimicrobial peptide RP-1, : 1 : 1 entities Detail

Experiments performed 3 experiments Detail